Nuclear retention of importin a coordinates cell fate through changes in gene expression
نویسندگان
چکیده
Yoshinari Yasuda, Yoichi Miyamoto, Tomoko Yamashiro, Munehiro Asally, Ayumi Masui, Chin Wong, Kate L Loveland and Yoshihiro Yoneda* Department of Frontier Biosciences, Graduate School of Frontier Biosciences, Osaka University, Osaka, Japan, Department of Biochemistry and Molecular Biology, School of Biomedical Sciences, Monash University, Clayton, Victoria, Australia, Department of Anatomy and Cell Biology, School of Biomedical Sciences, Monash University, Clayton, Victoria, Australia, Australian Research Council Centre of Excellence in Biotechnology and Development, Monash University, Clayton, Victoria, Australia, Department of Biochemistry, Graduate School of Medicine, Osaka University, Osaka, Japan and CREST, JST, Graduate School of Frontier Biosciences, Osaka University, Osaka, Japan
منابع مشابه
Nuclear retention of importin α coordinates cell fate through changes in gene expression.
Various cellular stresses including oxidative stress induce a collapse of the Ran gradient, which causes accumulation of importin α in the nucleus and a subsequent block of nuclear protein import. However, it is unknown whether accumulated importin α performs roles in the nucleus after its migration in response to stress. In this study, we found that nuclear-retained importin α2 binds with DNas...
متن کاملNuclear importin α and its physiological importance
Importin α is recognized as a classical nuclear localization signal (cNLS) receptor which mediates nucleocytoplasmic transport. However, it rapidly accumulates in the nucleus in response to cellular stresses, including oxidative stress, causing a blockade of the classical nuclear import pathway. We set out to determine whether importin α performs roles in the nucleus after cellular exposure to ...
متن کاملThe role of Importin-βs in the maintenance and lineage commitment of mouse embryonic stem cells☆
Members of the Importin-β family recognize nuclear localization signals (NLS) and nuclear export signals (NES). These proteins play important roles in various nucleocytoplasmic transport processes in cells. Here, we examined the expression patterns of 21 identified Importin-β genes in mouse embryonic stem cells (mESCs), mouse embryonic fibroblast (MEF) and mESCs differentiated into neural ectod...
متن کاملI-12: Nuclear Reprogramming in Bovin Somatic Cell Nuclear Transfer
Somatic cell nuclear transfer (SCNT or cloning) returns a differentiated cell to a totipotent status; a process termed nuclear reprogramming. Reproductive cloning has potential applications in both agriculture and biomedicine, but is limited by low efficiency. To understand the deficiencies of nuclear reprogramming, our research has focused on both candidate genes and global gene expression pat...
متن کاملPutting things in place for fertilization: discovering roles for importin proteins in cell fate and spermatogenesis
Importin proteins were originally characterized for their central role in protein transport through the nuclear pores, the only intracellular entry to the nucleus. This vital function must be tightly regulated to control access by transcription factors and other nuclear proteins to genomic DNA, to achieve appropriate modulation of cellular behaviors affecting cell fate. Importin-mediated nucleo...
متن کامل